The tool pictured above maintains cryogenically frozen specimens at an operator-selected ultralow temperature while the specimen’s frozen water is replaced with a suitable resin and the resin is solidified. The specimen is then warmed to room temperature for subsequent thin sectioning. This process, following cryo-fixation, can best preserve the hydrated appearance, structure, chemistry, and/or function (e.g. immunological properties) of microscopy specimens. (Image: Reichert-Jung CS auto cryo substitution system).